Calcium and Osmoregulation

[Mz RO]

[Dataguru]

Clinical:Ringer's (Sodium chloride, potassium chloride and calcium chloride)
http://wiki.medpedia.com/Clinical:Ringe ... hloride%29

Each 100 mL of Ringer's Injection USP contains:
Sodium Chloride USP 0.86 g; Calcium Chloride Dihydrate USP 0.033 g Potassium Chloride USP 0.03 g; Water for Injection USP qs
pH: 5.8 (5.0–7.5) Calculated Osmolarity: 310 mOsmol/liter
Concentration of Electrolytes (mEq/liter): Sodium 147; Potassium 4 Calcium 4.5; Chloride 156

Ingredients Molecular Formula Molecular Weight
Sodium Chloride USP NaCl 58.44
Potassium Chloride USP KCl 74.55
Calcium Chloride Dihydrate USP CaCl2•2H2O 147.02

http://www.springerlink.com/content/d62w8u18r0247254/
journal article that might have normal concentrations for Cyprinus carpio var. communis

Humans
http://www.surgeryencyclopedia.com/Ce-F ... Tests.html
-serum or plasma sodium: 135–145 mmol/l; alert levels: less than 120 mmol/l and greater than 160 mmol/l
-serum potassium: 3.6–5.4 mmol/l (plasma, 3.6–5.0 mmol/l); alert levels: less than 3.0 mmol/l and greater than 6.0 mmol/l
-serum or plasma chloride: 98–108 mmol/l
-serum or plasma bicarbonate: 18–24 mmol/l (as total carbon dioxide, 22–26 mmol/l); alert levels: less than 10 mmol/l and greater than 40 mmol/l
-serum calcium: 8.5–10.5 mg/dl (2.0–2.5 mmol/l); alert levels: less than 6.0 mg/dl and greater than 13.0 mg/dl
-ionized calcium: 1.0–1.3 mmol/l
-serum inorganic phosphorus: 2.3–4.7 mg/dl (children, 4.0–7.0 mg/dl); alert level: less than 1.0 mg/dl
-serum magnesium: 1.8–3.0 mg/dl (1.2–2.0 meq/l or 0.5–1.0 mmol/l)
-ionized magnesium: 0.53–0.67 mmol/l
-osmolality (calculated) 280–300 mosm/kg

[Dataguru]

From Fish Pathology, by Ronald J. Roberts, 2001
wolf 1963
in fish blood, in mg/100ml
cl 424
na 358
K 20.1
ca 12.5
phosphorus 9.3
mg 2.3
sulfate .8

[Dataguru]

ok. let's see if I can calculate the amounts to add to the water to match the blood concentrations.
The above mg/100ml convert to
42.4 mg/l Cl
35.8 mg/l Na
2 mg/l K
1.3 mg/l Ca
0.9 mg/l P
0.2 mg/l Mg
0.1 mg/l SO4

one gram of
sodium Chloride (NaCl) has 607mg Cl and 393 mg Na
Calcium Chloride (CaCl2 2H2O) has 311 mg Ca and 550mg Cl
Mag sulfate (MGSO4 7H2O) has 99mg Mg and 390mg SO4
Potassiun chloride (KCl) 524mg K and 476mg Cl

so adding a gram of each to 1 liter of water would result in the ppm above
sodium Chloride (NaCl) has 607mg/l Cl and 393 mg/l Na
Calcium Chloride (CaCl2 2H2O) has 311 mg/l Ca and 550mg/l Cl
Mag sulfate (MGSO4 7H2O) has 99mg/l Mg and 390mg/l SO4
Potassiun chloride (KCl) 524mg/l K and 476mg/l Cl

I want it in gallons and 1 gallon of water is 3.78541178 liters
so adding a gram of each to 1 gallon of water would result in
sodium Chloride (NaCl) has 160mg/l Cl and 104 mg/l Na
Calcium Chloride (CaCl2 2H2O) has 82 mg/l Ca and 145mg/l Cl
Mag sulfate (MGSO4 7H2O) has 26mg/l Mg and 103mg SO4
Potassiun chloride (KCl) 139mg K and 126mg Cl

So if I want to match the blood levels, I'd need to add per gallon
for Na, I'd need to add 34% of that amount (345mg NaCl)
for Ca, I'd need to add 49% of that amount (488mg CaCl)
for Mg, I'd need to add 93% of that amount (933mg MgSO4)
for K, I'd need to add 28% of that amount (282mg KCl)

That would result in
161.6ppm Cl (desired 42.4ppm)
35.8ppm Na (desired 35.8ppm)
40.1ppm Ca (desired 40.1ppm)
24.3ppm Mg (desired 24.3ppm)
39.1ppm K (desired 39.1ppm)
96.1ppm SO4 (desired 0.1ppm)

that amount of calcium and magnesium should raise GH by 100ppm each.

Anyone care to double check my calculations?

[Dataguru]

So if that is correct, given I added 1 tsp salt per gallon and about 1.3 grams of calcium chloride per gallon
cl 993.5ppm
na 519.6ppm
ca 108.7ppm